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Image Search Results
Journal: PLoS ONE
Article Title: A Rapid and Low-Cost PCR Thermal Cycler for Infectious Disease Diagnostics
doi: 10.1371/journal.pone.0149150
Figure Lengend Snippet: Target, primer sequences, target sizes, and components of PCR.
Article Snippet: Human DNA (600 bp, 500 bp, 400 bp, 300 bp, 200 bp, 100 bp) , 7.5 μL
Techniques: Amplification, One Step RT-PCR, Virus
Journal: Microorganisms
Article Title: The Absence of C-5 DNA Methylation in Leishmania donovani Allows DNA Enrichment from Complex Samples
doi: 10.3390/microorganisms8081252
Figure Lengend Snippet: Enrichment (X) of Leishmania DNA in artificial mixtures of Leishmania promastigote DNA and human DNA, with the mixtures ranging from 1:15 to 1:15,000 Leishmania: human DNA. Enrichments were carried out with the NEBNext Microbiome DNA Enrichment Kit (NEB), and the unmethylated Leishmania DNA was enriched on average 263 times.
Article Snippet: To check whether the lack of detectable DNA methylation in Leishmania can be used for the enrichment of Leishmania vs. (methylated) human DNA, we carried out methylated DNA removal on two types of samples: (1) An artificial mix of L. donovani BPK282/0 cl4 promastigote DNA with
Techniques:
Journal: Microorganisms
Article Title: The Absence of C-5 DNA Methylation in Leishmania donovani Allows DNA Enrichment from Complex Samples
doi: 10.3390/microorganisms8081252
Figure Lengend Snippet: Enrichment (X) of Leishmania DNA in artificial mixtures of Leishmania and human DNA for promastigotes and amastigotes of 3 clinical isolates (BPK026, BPK275, and BPK282). Enrichments were carried out with the NEBNext Microbiome DNA Enrichment Kit (NEB).
Article Snippet: To check whether the lack of detectable DNA methylation in Leishmania can be used for the enrichment of Leishmania vs. (methylated) human DNA, we carried out methylated DNA removal on two types of samples: (1) An artificial mix of L. donovani BPK282/0 cl4 promastigote DNA with
Techniques:
Journal: bioRxiv
Article Title: The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samples
doi: 10.1101/747063
Figure Lengend Snippet: Enrichment (X) of Leishmania DNA in artificial mixtures of Leishmania promastigote DNA and human DNA, with the mixtures ranging from 1:15 to 1:15000 Leishmania: human DNA. Enrichments were carried out with the NEBNext Microbiome DNA Enrichment Kit (NEB) and the unmethylated Leishmania DNA was enriched on average 263 times.
Article Snippet: For this experiment, we used a 1/1500 artificial mix of
Techniques: